Journal: Neuropsychiatric Disease and Treatment

Article Title: Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides

doi: 10.2147/NDT.S145069

Figure Lengend Snippet: Artesunate prevented rats from the clozapine-induced hepatic fibrosis. Notes: Rats were intraperitoneally given saline, clozapine, artesunate, or clozapine + artesunate for 6 weeks. At the end, the rats were deeply anesthetized with sodium pentobarbital and liver sample was taken and processed for immunohistochemical staining with the primary antibody against α-SMA. Representative images were from saline group ( A ), clozapine group ( B ), artesunate group ( C ), and clozapine + artesunate group ( D ) rats. The arrows in images point to positive α-SMA immunos-taining. 200× magnification. Abbreviation: α-SMA, α-smooth muscle actin.

Article Snippet: Immunohistochemical staining was performed using the primary antibody against α-smooth muscle actin (α-SMA) and an immunohistochemical assay kit (SV0001-mouse IgG) from Boster (Wuhan, China) to examine the activation of hepatic stellate cells and hepatic fibrosis/cirrhosis of rats.

Techniques: Saline, Immunohistochemical staining, Staining

Journal: The Journal of international medical research

Article Title: Physical properties of poly(N-isopropylacrylamide) hydrogel promote its effects on cardiac protection after myocardial infarction.

doi: 10.1177/030006051204000615

Figure Lengend Snippet: FIGURE 6: Neovasculature formation in sham-operated and infarct areas of myocardium 90 days after induction of myocardial infarction (MI) in rats, as identified by immunohistochemical staining for α-smooth muscle actin. (A) Sham-operated group; (B) phosphate-buffered saline (PBS)-treated MI group; (C) Gel A-treated MI group; (D) Gel B-treated MI group (scale bars, 100 µm). Immunohistochemical staining was used to quantify (E) blood vessel density per high-power field (HPF) in each group. Black arrows show neovasculature formed in sham-operated or infarcted myocardium. aP < 0.05 compared with sham-operated group; bP < 0.05 compared with MI + PBS group; one-way analysis of variance and unpaired Student’s t-test

Article Snippet: The slides were incubated overnight at 4 °C with anti-αsmooth muscle actin primary antibody (dilution 1 : 50; Boster, Wuhan, China).

Techniques: Immunohistochemical staining, Staining, Saline

Journal: iScience

Article Title: DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation

doi: 10.1016/j.isci.2021.103086

Figure Lengend Snippet:

Article Snippet: ACTA2 , GeneTex , Cat#GTX124505; RRID: AB_11166745.

Techniques: Enzyme-linked Immunosorbent Assay, RNA Extraction, SYBR Green Assay, Recombinant, Cell Culture, Sterility, Modification, Multiplex sample analysis, Labeling, Reverse Transcription, Purification, Ab Array, Bicinchoninic Acid Protein Assay, Protein Extraction, Reporter Assay, Sequencing, shRNA, Software